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addgene 43802  (Addgene inc)


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    Structured Review

    Addgene inc addgene 43802
    Addgene 43802, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cas9/pmc12856427-1-10-10?v=Addgene+inc
    Average 93 stars, based on 119 article reviews
    addgene 43802 - by Bioz Stars, 2026-06
    93/100 stars

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    Image Search Results


    Immunization experiment scheme for in vivo screen Host mice (IOMA gl) were immunized with OVA in alum. At week 2 after immunization, B cells from B1-8 hi , LSL-Cas9, AID-Cre, Kappa KO animals were transduced with pooled sgRNA library retrovirus and adoptively transferred to the hosts. Subsequently, the host animals were boosted with NP-OVA in alum at day 0 and 2 and administered with anti-DEC205-OVA mAb at day 6.5. Spleens were harvested and processed for cell sorting at day 10.

    Journal: STAR Protocols

    Article Title: Protocol for an in vivo CRISPR screen for germinal center B cells in mice using ecotropic retrovirus

    doi: 10.1016/j.xpro.2026.104586

    Figure Lengend Snippet: Immunization experiment scheme for in vivo screen Host mice (IOMA gl) were immunized with OVA in alum. At week 2 after immunization, B cells from B1-8 hi , LSL-Cas9, AID-Cre, Kappa KO animals were transduced with pooled sgRNA library retrovirus and adoptively transferred to the hosts. Subsequently, the host animals were boosted with NP-OVA in alum at day 0 and 2 and administered with anti-DEC205-OVA mAb at day 6.5. Spleens were harvested and processed for cell sorting at day 10.

    Article Snippet: Mouse: 6-weeks-old, sex-matched LSL-Cas9: B6J.129(B6N)-Gt(ROSA)26Sortm1(CAG-cas9∗,-EGFP)Fezh/J , The Jackson Laboratory , cat# 026175.

    Techniques: In Vivo, Transduction, FACS